Detection of virulence iroN gene in Klebsiella pneumoniae in urinary tract infections patients from Iraq

Abstract

Klebsiella pneumoniae belongs to the family Enterobacteriaceae, and it is the most clinically pertinent species, also it causes both nosocomial and communities-acquired infections, such as liver abscesses, urinary infections, pneumonia, and bacteremia. The current study aimed to detect the virulence iroN gene in Klebsiella pneumoniae for patients with urinary tract infections. One hundred fifty collected 75 samples of urine and 75 samples of blood samples from patients with urinary tract infections from Ibn Al-Baladi Hospital/ Baghdad -Iraq from November 2023 to February 2024. The results showed the diagnosis of 60 isolates of bacteria with an 80% prevalence of Klebsiella pneumoniae observed percentage for the age (more than 60 years) was 80% higher than for age (less than 60 years) was 20%. Moreover, females recorded 55% and males 45%. Also, blood group O has a percentage (33.3% ) more than other groups A (23.3%), AB(30%), and B (13.3%). The results of antibiotic resistance of Klebsiella pneumoniae showed 100% resistance toward Cefoxitin Screen, Amoxicillin/ Clavulanic acid, Ticarcillin/ Clavulanic Acid, Piperacillin, Piperacillin/Tazobactam, Cefmetazole, Ceftazidim, Cefepime, Aztreonam, Amikacin, Ciprofloxacin). In contrast, it was 63.3 % for (Meropenem, Gentamicin, Tobramycin),66.7%for Trimethoprim / Sulfamethoxazole, and 61.6% for Imipenem. Moreover, these isolates were sensitive to Ticarcillin (100%), Minocycline (83%). Besides, the results of the virulence gene showed iroN gene was found in 38(63.3%) isolates out of 60 isolates. In conclusion, this study appeared to the prevalence resistance of K. pneumoniae in urinary infections with the presence of iroN gene.