Molecular detection of some Vancomycin Pathogenic bacteria in Basrah Governorate

Abstract

The current study aimed to study the resistance of some species Bacterial resistance to the antibiotic vancomycin. 121 samples were collected and grown on Chocolate Agar medium for isolating pathogenic bacteria. and then It was grown on Nutrient Agar medium for pure colonies. These bacteria were diagnosed using phenotypic methods, biochemical tests, and 16SrRNA gene. The results reported that 40 (33.3%) resistant strains appeared, of which 13 were males with a percentage of (32.5%), while the number of female samples was 27 with a percentage (67.5%) and the rest were sensitive to this antibiotic. done during the study The current study obtained four types of bacteria resistant to vancomycin: 24 strains (60%) belonging to Pseudomonas aeruginosa bacteria, 11 strains (27.5%) belonging to Proteus mirabilis bacteria, 4 strains (10%) of Staphylococcus aureus bacteria, and one strain (2.5%) for Actinomyces sp. As for the presence of resistance genes, Vancomycin, where the current study recorded the presence of 17 bacterial strains containing both VanA and VanB genes (42.5%), while the VanA gene was present in 8 strains (20%), and the VanB gene was present in 6 strains (15%), and 9 appeared Strains (22.5%) do not possess either of the two genes (VanA and VanB), although they were resistant to vancomycin, and this may be due to their possession of other resistance genes or other mechanisms of resistance to this antibiotic.