Affiliation:
1. Université Mustapha Stambouli de Mascara
2. Yıldız Technical University
3. Aksaray University
4. University Hospital of Sidi-Bel-Abbes
Abstract
This study aimed to evaluate the antigastroenteritis effect against
Salmonella enterica subsp. arizonae after therapeutic application of
hydromethanolic extracts (MeOH.E) and aqueous extracts (Aq.E) of Zizyphus
lotus (ZL) and Ruta chalepensis (RC). Acute oral toxicity was elucidated
using in vivo methods and antigastroenteritis effect were evaluated using
S. enterica subsp. arizonae-induced diarrheal model. Furthermore, test
groups were treated with 400 mg/kg of the MeOH.E and Aq.E of each plant,
while the control group was given neomycin (200 mg/kg) as standard
antibiotic treatment, positive and negative controls were given the
infectious germ (4 × 106 cells/mL) and 0.9% saline solution NaCl (10
ml/kg), respectively. Both plants extracts showed no toxicity for all the
animals, so the LD50 was found to be greater than 5000 mg/kg. Moreover, an
important bactericidal effect, using both plants extracts was determined
against S. enterica subsp. arizonae cells in the intestine. In parallel, a
decrease in alkaline phosphatase, amino alanine transferase and aspartate
aminotransferase levels was observed with reduction in blood erythrocyte
sedimentation rate in all treated animals. Thus, these results could be
exploited in the medical field for the formulation of potent antibacterial
drugs that cure severe gastrointestinal infections.
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology