Affiliation:
1. College of Chemistry and Pharmaceutical Engineering, Sichuan University of Science and Engineering, Zigong, Sichuan 643000, P.R. China
2. College of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, P.R. China
Abstract
An azamacrocyclic compound with a carboxyl branch, 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclo-tetradecane- N/-acetic acid (L), and its cerium complex were synthesised and characterised. The mode of combination of the cerium complex with DNA was investigated by fluorescence and UV-Vis absorption spectroscopy. The cutting function of the cerium complex towards supercoiled DNA was studied by the gel electrophoresis method. The results show that metal complex can bind to the phosphate group of DNA double helix and promote the hydrolysis of the phosphodiester bond of supercoiled DNA (Form I); under certain conditions, supercoiled form DNA (Form I) was transformed into the nicked form DNA (Form II) under the strong cutting effect of the macrocyclic cerium complex; the cutting is completed by a hydrolysis mechanism.
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4 articles.
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