Affiliation:
1. National Organization for Drug Control and Research (NODCAR) , Giza, Egypt
2. Ain Shams University, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department , Abbassia, Cairo 11566, Egypt
Abstract
Abstract
Background
Green Fourier transform infrared (FTIR) spectroscopy, using potassium bromide (KBr) disc technique, eliminates the consumption of hazardous chemicals. Spectrofluorimetry for drugs that have native fluorescence. Mean centering of ratio spectra (MCRS) analysing overlapped spectra without preliminary separation.
Objective
Three simple, accurate, and sensitive methods have been developed and validated for the determination of canagliflozin (CANA); one is a stability-indicating method for CANA and metformin (MET) determination.
Methods
Method A is FTIR using a KBr disc for CANA determination measuring alkyl halide C–F peak area centered on 1230/cm. Method B is spectrofluorimetry using Δ λ = 50 nm synchronous mode at a peak maximum of 291.8 nm for CANA determination using methanol as solvent. Method C is a stability-indicating MCRS method measuring the peak amplitude of CANA and MET at 306.2 and 246.6 nm, respectively, in their mixture with complete CANA oxidation degradation.
Results
The linear ranges were: FTIR spectroscopy, 1.50–24.70 μg/mg CANA; spectrofluorimetry, 100.00–600.00 ng/mL CANA; and MCRS, 1.00–25.00 μg/mL CANA and 1–30 μg/mL MET.
Conclusion
All results were statistically compared with a reported method: no significant difference was observed.
Highlights
The proposed methods can be used efficiently for routine analysis in QC laboratories. A green FTIR method utilizes only one reagent, KBr. Spectrofluorimetry using a constant wavelength synchronous scan of CANA native fluorescence in nanogram concentrations overcomes conventional excitation/emission spectra drawbacks and proves that the solvent effect in the fluorescence intensity differs according to concentration used. Stability-indicating MCRS determines the studied drugs in bulk, pharmaceutical formulations, accelerated and long-term stability study samples.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
1 articles.
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