Optimization of Extraction and Quality Evaluation of Abri Herba by Response Surface Methodology Combined with Quantitative Analysis of Multi-Components by a Single Marker

Author:

Shen Yajun12ORCID,Li Hengyang12ORCID,Ding Xiaoying12ORCID,Zhan Zhilai3ORCID,An Qi14ORCID,Zheng Yuguang15ORCID,Zhang Dan12ORCID

Affiliation:

1. Hebei University of Chinese Medicine, College of Pharmacy, Traditional Chinese Medicine Processing Technology Innovation Centre of Hebei Province , Shijiazhuang 050200, China

2. Hebei University of Chinese Medicine, College of Pharmacy, International Joint Research Centre on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province , Shijiazhuang 050200, China

3. China Academy of Chinese Medical Sciences, National Resource Centre for Chinese Materia Medica , Beijing 100700, China

4. Hebei Institute for Drug and Medical Device Control, Department of Chinese Materia Medica , Shijiazhuang 050200, China

5. Hebei Chemical and Pharmaceutical College, Department of Pharmaceutical Engineering , Shijiazhuang 050026, China

Abstract

Abstract Background Abri Herba (AH) is a famous medicinal and edible traditional Chinese medicine, which is usually used for liver disease. To date, few studies have been conducted on the ultrasonic extraction (UAE) process for AH and the application of quality analysis of multi-components by the single-marker (QAMS) method to evaluate the quality. Objective To optimize the UAE process for AH, and develop and validate the quality evaluation of AH by the QAMS method. Methods The UAE conditions of AH were optimized by response surface methodology with the total contents of protocatechuic acid, hydantoin, gardeniaine, vicenin-2, salvoside and isosalvoside as indicators, the ultrasonic time, methanol concentration and liquid to material ratio as parameters. The content of protocatechuic acid, hypaphorine, abrine, vicenin-2, schaftoside, and isoschaftoside in 12 batches of AH was first determined by the external standard method (ESM) using HPLC. After that, based on abrine as the internal standard, the relative correction factors (RCF) for protocatechuic acid, hypaphorine, vicenin-2, schaftoside and isoschaftoside were established, and the ESM method was used to verify the QAMS method. Results The results show that the optimal extraction process parameters for AH are an ultrasonic time of 22 min, a methanol concentration of 45%, and liquid to material ratio of 26 (mL/g). The QAMS results show that the relative correction factor has good reproducibility, and there is no significant difference between the results of the ESM method and the QAMS method for each chemical constituent, indicating that the research is feasible. Conclusion The optimized extraction process of AH and the established QAMS-based quality control method are stable and can be used for the quality control of AH. Highlights A response surface methodology was used to optimize the ultrasonic extraction process for AH, and a QAMS method was established for evaluating the quality of AH.

Funder

Natural Science Foundation of Hebei Province

“333 Talent Project” of Hebei Province

Ability Establishment of Sustainable Use for Valuable Chinese Materia Medica Resources

Scientific Research Programme of Hebei Provincial Administration of Traditional Chinese Medicine

Innovation Team of Hebei Province Modern Agricultural Industry Technology System

Key R&D plan of Hebei Province

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

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