Affiliation:
1. Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College , Nangchang, Jiangxi 330004, China
2. Key Laboratory of Modern Preparation of TCM, Jiangxi University of Traditional Chinese Medicine , Nangchang, Jiangxi 330004, China
3. Research Center of Chinese Herbal Medicine and Ethnic Medicine , Nanchang, Jiangxi 330004, China
Abstract
Abstract
Background
The vine stem of Spatholobus suberectus Dunn (S. suberectus), called “JiXueTeng”, has been used as a significant medicine for thousands of years in China. However, reliable field identification of this medicinal plant remains problematic, inaccurate identification may cause serious adverse effects in the functions of the drug and may affect the clinical medication reviews.
Objective
To ensure use of the exact medicine and implement protective legislation, it is imperative to obtain the chloroplast (cp) genome of S. suberectus, which can be used as a valuable resource for species identification and phylogenetic analysis.
Methods
In this study, the complete cp genomes of S. suberectus (152 173 bp (base pair)) and S. pulcher (151 099 bp) were assembled for the first time by using next-generation sequencing (NGS) technology to gain abundant information on the genus of Spatholobus. And some bioinformatics softwares were used for data filtering, assembling and analyzing.
Results
We found the G and C contents of S. suberectus and S. pulcher were close, 35.19% and 35.37%, respectively. The noncoding regions were more divergent than coding ones. Moreover, we revealed eight divergence hotspots (trnH, trnK-rbcL, trnL-rbcT, psbD-trnT, trnC-rpoB, atpI-atpH, ycf4, and trnL-rpl32) which might be used as candidate molecular markers for Spatholobus identification. The analysis of the phylogenetic relationship indicated that two Spatholobus species were clustered together and two Spatholobus species was sister to the Cajanus.
Conclusion
The findings of this study were conducive to species identification and phylogenetic research of Spatholobus and provided valuable resources for finding the substitution of S. suberectus.
Highlights
We assembled the complete cp genomes of S. suberectus and S. pulcher for the first by using next-generation sequencing.
Funder
National Natural Science Foundation of China
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry