Hydrolysis of Proteins for Amino Acid Analysis. II. Acid Hydrolysis in Sealed Tubes of Mixtures of β-Lactoglobulin and Starch

Abstract

Abstract Experiments with a mixture of β-Iactoglobulin and starch, simulating the composition of carbohydrate-rich foods and feeds, have been carried out to determine conditions for acid hydrolysis that will permit maximal recovery of amino acids in hydrolysates of such materials. When a large excess of 6N HC1 is used for hydrolysis, good recoveries of most amino acids are obtained. However, about one-quarter of the tyrosine present is destroyed under the conditions investigated. Some destruction of methionine and cystine may also be attributed to the presence of carbohydrate, but a special method for the determination of these amino acids is available.