Cephalopod-omics: Emerging Fields and Technologies in Cephalopod Biology

Author:

Baden Tom1ORCID,Briseño John2ORCID,Coffing Gabrielle3ORCID,Cohen-Bodénès Sophie4ORCID,Courtney Amy5ORCID,Dickerson Dominick6ORCID,Dölen Gül7ORCID,Fiorito Graziano8ORCID,Gestal Camino9ORCID,Gustafson Taryn10,Heath-Heckman Elizabeth11ORCID,Hua Qiaz12ORCID,Imperadore Pamela8ORCID,Kimbara Ryosuke13ORCID,Król Mirela14,Lajbner Zdeněk15ORCID,Lichilín Nicolás16ORCID,Macchi Filippo17ORCID,McCoy Matthew J18ORCID,Nishiguchi Michele K19ORCID,Nyholm Spencer V2ORCID,Otjacques Eve2021ORCID,Pérez-Ferrer Pedro Antonio19ORCID,Ponte Giovanna8ORCID,Pungor Judit R3ORCID,Rogers Thea F16ORCID,Rosenthal Joshua J C22ORCID,Rouressol Lisa16ORCID,Rubas Noelle23ORCID,Sanchez Gustavo24ORCID,Santos Catarina Pereira20,Schultz Darrin T16ORCID,Seuntjens Eve25ORCID,Songco-Casey Jeremea O3ORCID,Stewart Ian Erik26ORCID,Styfhals Ruth25ORCID,Tuanapaya Surangkana27ORCID,Vijayan Nidhi2ORCID,Weissenbacher Anton28,Zifcakova Lucia15ORCID,Schulz Grace29,Weertman Willem30,Simakov Oleg16ORCID,Albertin Caroline B22ORCID

Affiliation:

1. School of Life Sciences, University of Sussex , Brighton BN1 9QG , UK

2. Molecular and Cell Biology Department, University of Connecticut, Storrs , CT 06269 , USA

3. Biology Department: Institute of Ecology and Evolution, University of Oregon, Eugene , OR 97403-5289 , USA

4. Laboratoire des Systèmes Perceptifs, Département d'Etudes Cognitives, Ecole Normale Supérieure, PSL University, CNRS , 75005 Paris , France

5. MRC Laboratory of Molecular Biology, Francis Crick Avenue , Cambridge CB2 0QH , UK

6. Friday Harbor Laboratory, University of Washington , Seattle, WA 98250 , USA

7. Department of Neuroscience, Johns Hopkins University , Baltimore, MD 21218 , USA

8. Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn , 80121 Napoli , Italy

9. Laboratory of Marine Molecular Pathobiology, Institute of Marine Research (IIM), Spanish National Research Council (CSIC) , Vigo 36208 , Spain

10. University of Central Florida , Orlando, FL 32826 , USA

11. Departments of Integrative Biology and Microbiology and Molecular Genetics, Michigan State University , East Lansing, MI 48824 , USA

12. Department of Ecology and Evolution, University of Adelaide , Adelaide, South Australia 5000 , Australia

13. Misaki Marine Biological Station, School of Science, The University of Tokyo , Miura, Kanagawa 238-0225 , Japan

14. Adam Mickiewicz University in Poznań , Poznań 61-712 , Poland

15. Physics and Biology Unit, Okinawa Institute of Science and Technology Graduate University , 1919-1 Tancha, Onna, Kunigami District, Okinawa 904-0495 , Japan

16. Department of Neurosciences and Developmental Biology, University of Vienna , Vienna 1010 , Austria

17. Program in Biology, New York University Abu Dhabi , P.O. Box 129188 Abu Dhabi , United Arab Emirates

18. Department of Pathology, Stanford University , Stanford, CA 94305 , USA

19. Department of Molecular and Cell Biology, School of Natural Sciences, University of California , Merced, 5200 N. Lake Blvd., Merced, CA 95343 , USA

20. MARE—Marine and Environmental Sciences Centre & ARNET—Aquatic Research Network, Laboratório Marítimo da Guia, Faculdade de Ciências, Universidade de Lisboa , Av. Nossa Senhora do Cabo, 939, 2750-374 Cascais , Portugal

21. Division of Biosphere Sciences and Engineering, Carnegie Institution for Science , 1200 E. California Blvd, Pasadena, CA 91125 , USA

22. Marine Biological Laboratory, The Eugene Bell Center for Regenerative Biology and Tissue Engineering , Woods Hole, MA 02543-1015 , USA

23. Department of Molecular Biosciences and Bioengineering, University of Hawaii Manoa , Honolulu, HI 96822 , USA

24. Molecular Genetics Unit, Okinawa Institute of Science and Technology Graduate University , Onna, Okinawa 904-0495 , Japan

25. Laboratory of Developmental Neurobiology, Department of Biology, KU Leuven , Leuven 3000 , Belgium

26. Neural Circuits and Behaviour Lab, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC) , Berlin 13125 , Germany

27. Laboratory of genetics and applied breeding of molluscs, Fisheries College, Ocean University of China , Qingdao 266100 , China

28. Vienna Zoo , Maxingstraße 13b, 1130 Vienna , Austria

29. University of Chicago , Chicago, IL 60637 , USA

30. Friday Harbor Laboratory, University of Washington, Seattle, WA 98250 , USA

Abstract

Synopsis Few animal groups can claim the level of wonder that cephalopods instill in the minds of researchers and the general public. Much of cephalopod biology, however, remains unexplored: the largest invertebrate brain, difficult husbandry conditions, and complex (meta-)genomes, among many other things, have hindered progress in addressing key questions. However, recent technological advancements in sequencing, imaging, and genetic manipulation have opened new avenues for exploring the biology of these extraordinary animals. The cephalopod molecular biology community is thus experiencing a large influx of researchers, emerging from different fields, accelerating the pace of research in this clade. In the first post-pandemic event at the Cephalopod International Advisory Council (CIAC) conference in April 2022, over 40 participants from all over the world met and discussed key challenges and perspectives for current cephalopod molecular biology and evolution. Our particular focus was on the fields of comparative and regulatory genomics, gene manipulation, single-cell transcriptomics, metagenomics, and microbial interactions. This article is a result of this joint effort, summarizing the latest insights from these emerging fields, their bottlenecks, and potential solutions. The article highlights the interdisciplinary nature of the cephalopod-omics community and provides an emphasis on continuous consolidation of efforts and collaboration in this rapidly evolving field.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Animal Science and Zoology

Reference138 articles.

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5. Cephalopod biology: at the intersection between genomic and organismal novelties;Albertin;Annu Rev Anim Biosci,2020

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