Overcoming confounding plate effects in differential expression analyses of single-cell RNA-seq data

Author:

Lun Aaron T. L.1,Marioni John C.23

Affiliation:

1. Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, RobinsonWay, Cambridge CB2 0RE, UK aaron.lun@cruk.cam.ac.uk

2. Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK

3. EMBL European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK and Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK marioni@ebi.ac.uk

Publisher

Oxford University Press (OUP)

Subject

Statistics, Probability and Uncertainty,General Medicine,Statistics and Probability

Reference27 articles.

1. Differential expression analysis for sequence count data.;Anders;Genome Biology,2010

2. Fitting linear mixed-effects models using lme4.;Bates;Journal of Statistical Software,2015

3. MAST: a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data.;Finak;Genome Biology,2015

4. On the widespread and critical impact of systematic bias and batch effects in single-cell RNA-Seq data.;Hicks,2015

5. Massively parallel single-cell RNA-seq for marker-free decomposition of tissues into cell types.;Jaitin;Science,2014

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