Salicylic Acid Induced Resistance in Drought-Stressed Pistachio Seedlings Influences Physiological Performance of Agonoscena pistaciae (Hemiptera: Aphalaridae)

Abstract

Abstract Induced host plant resistance is a potential approach to insect and disease management. Salicylic acid (SA) acts as a signal molecule to induce resistance in plants against sap-sucking insects. The effects of salicylic acid-induced resistance against common pistachio psylla, Agonoscena pistaciae Burckhardt and Lauterer, were investigated in well-watered and drought-stressed pistachio, Pistacia vera L. cv. Akbari, seedlings. Agonoscena pistaciae exhibited a significant preference for plants treated with SA as compared with untreated controls or those subjected to drought stress. Plants subjected to both drought stress and SA treatment were equivalently colonized as compared with control plants but were more attractive than those subjected to drought stress alone. Psyllid mortality increased on plants subjected to simultaneous drought stress and SA treatment as compared with controls. Salicylic acid treatment mediated production of defensive enzymes in plants, including superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol peroxidase (GPX) and polyphenol oxidase (PPO), as well as that of other metabolites such as phenol, malondialdehyde (MDA), H2O2, free amino acids, and pigments via phenylpropanoid pathways under conditions of drought. Despite increases in activity of detoxification (glutathione S transferase, carboxylesterase) and antioxidative (SOD, CAT, APX, phenoloxidase, GPX) enzymes in psyllids, reduced survival of A. pistaciae on drought stressed and SA-treated plants was likely caused by excessive H2O2 and high phenolic content in treated plants. Based on our results, we postulate that salicylic acid-induced defense against A. pistaciae under drought conditions could be manipulated to enhance antibiosis against this key pest in pistachio orchards.