Affiliation:
1. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University , Guangzhou 510631, China
Abstract
Abstract
In eukaryotes, the STRUCTURAL MAINTENANCE OF CHROMOSOME 5/6 (SMC5/6) complex is critical to maintaining chromosomal structures around double-strand breaks (DSBs) in DNA damage repair. However, the recruitment mechanism of this conserved complex at DSBs remains unclear. In this study, using Arabidopsis thaliana as a model, we found that SMC5/6 localization at DSBs is dependent on the protein scaffold containing INVOLVED IN DE NOVO 2 (IDN2), CELL DIVISION CYCLE 5 (CDC5), and ALTERATION/DEFICIENCY IN ACTIVATION 2B (ADA2b), whose recruitment is further mediated by DNA-damage-induced RNAs (diRNAs) generated from DNA regions around DSBs. The physical interactions of protein components including SMC5–ADA2b, ADA2b–CDC5, and CDC5–IDN2 result in formation of the protein scaffold. Further analysis indicated that the DSB localization of IDN2 requires its RNA-binding activity and ARGONAUTE 2 (AGO2), indicating a role for the AGO2–diRNA complex in this process. Given that most of the components in the scaffold are conserved, the mechanism presented here, which connects SMC5/6 recruitment and small RNAs, will improve our understanding of DNA repair mechanisms in eukaryotes.
Funder
Major Program of Guangdong Basic and Applied Research
National Natural Science Foundation of China
Natural Science Foundation of Guangdong
Guangdong Modern Agro-industry Technology Research System
China Postdoctoral Science Foundation
Program for Changjiang Scholars
Guangdong Special Support Program of Young Top-Notch Talent in Science and Technology Innovation
Pearl River Talent Plan
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Plant Science
Cited by
8 articles.
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