The Physcomitrium (Physcomitrella) patens PpKAI2L receptors for strigolactones and related compounds function via MAX2-dependent and -independent pathways

Author:

Lopez-Obando Mauricio123ORCID,Guillory Ambre1ORCID,Boyer François-Didier4ORCID,Cornu David5ORCID,Hoffmann Beate1ORCID,Le Bris Philippe1ORCID,Pouvreau Jean-Bernard6ORCID,Delavault Philippe6ORCID,Rameau Catherine1ORCID,de Saint Germain Alexandre1ORCID,Bonhomme Sandrine1ORCID

Affiliation:

1. Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France

2. Department of Plant Biology, Swedish University of Agricultural Sciences, The Linnean Centre for Plant Biology in Uppsala, SE-750 07 Uppsala, Sweden

3. VEDAS Corporación de Investigación e Innovación (VEDASCII), 050024 Medellín, Colombia

4. Institut de Chimie des Substances Naturelles, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, France

5. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, France

6. Laboratoire de Biologie et Pathologie Végétales, LBPV, Université de Nantes, 44000 Nantes, France

Abstract

Abstract In angiosperms, the α/β hydrolase DWARF14 (D14), along with the F-box protein MORE AXILLARY GROWTH2 (MAX2), perceives strigolactones (SL) to regulate developmental processes. The key SL biosynthetic enzyme CAROTENOID CLEAVAGE DIOXYGENASE8 (CCD8) is present in the moss Physcomitrium patens, and PpCCD8-derived compounds regulate moss extension. The PpMAX2 homolog is not involved in the SL response, but 13 PpKAI2LIKE (PpKAI2L) genes homologous to the D14 ancestral paralog KARRIKIN INSENSITIVE2 (KAI2) encode candidate SL receptors. In Arabidopsis thaliana, AtKAI2 perceives karrikins and the elusive endogenous KAI2-Ligand (KL). Here, germination assays of the parasitic plant Phelipanche ramosa suggested that PpCCD8-derived compounds are likely noncanonical SLs. (+)-GR24 SL analog is a good mimic for PpCCD8-derived compounds in P. patens, while the effects of its enantiomer (−)-GR24, a KL mimic in angiosperms, are minimal. Interaction and binding assays of seven PpKAI2L proteins pointed to the stereoselectivity toward (−)-GR24 for a single clade of PpKAI2L (eu-KAI2). Enzyme assays highlighted the peculiar behavior of PpKAI2L-H. Phenotypic characterization of Ppkai2l mutants showed that eu-KAI2 genes are not involved in the perception of PpCCD8-derived compounds but act in a PpMAX2-dependent pathway. In contrast, mutations in PpKAI2L-G, and -J genes abolished the response to the (+)-GR24 enantiomer, suggesting that PpKAI2L-G, and -J proteins are receptors for moss SLs.

Funder

the Agence Nationale de la Recherche

the Labex Saclay Plant Sciences-SPS

“Infrastructures en Biologie Santé et Agronomie”

SICAPS platform of the Institute for Integrative Biology of the Cell, and CHARM3AT Labex program

AgreenSkills award from the European Union in the framework of the Marie-Curie FP7 COFUND People Programme and fellowship from Saclay Plant Sciences

I2BC proteomic platform (Proteomic-Gif, SICaPS) supported by IBiSA, Ile de France Region, Plan Cancer, CNRS and Paris-Sud University

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Plant Science

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