Affiliation:
1. Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, CNRS, INRAE, 69342 Lyon, France
2. LYMIC-PLATIM imaging and microscopy core facility, Univ Lyon, SFR Biosciences, ENS de Lyon, Inserm US8, CNRS UMS3444, UCBL-50 Avenue Tony Garnier, 69007 Lyon, France
Abstract
Abstract
At the center of cell biology is our ability to image the cell and its various components, either in isolation or within an organism. Given its importance, biological imaging has emerged as a field of its own, which is inherently highly interdisciplinary. Indeed, biologists rely on physicists and engineers to build new microscopes and imaging techniques, chemists to develop better imaging probes, and mathematicians and computer scientists for image analysis and quantification. Live imaging collectively involves all the techniques aimed at imaging live samples. It is a rapidly evolving field, with countless new techniques, probes, and dyes being continuously developed. Some of these new methods or reagents are readily amenable to image plant samples, while others are not and require specific modifications for the plant field. Here, we review some recent advances in live imaging of plant cells. In particular, we discuss the solutions that plant biologists use to live image membrane-bound organelles, cytoskeleton components, hormones, and the mechanical properties of cells or tissues. We not only consider the imaging techniques per se, but also how the construction of new fluorescent probes and analysis pipelines are driving the field of plant cell biology.
Funder
European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme
ANR caLIPSO
ANR STAYING-TIGHT
ANR PLANTSCAPE
ANR ChromAuxi
French Ministry of Higher Education
CNRS/University of Melbourne network
ENS de Lyon and from the BAP department of INRAE
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Plant Science
Cited by
21 articles.
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