Imaging the living plant cell: From probes to quantification

Author:

Colin Leia1ORCID,Martin-Arevalillo Raquel1ORCID,Bovio Simone12ORCID,Bauer Amélie1ORCID,Vernoux Teva1ORCID,Caillaud Marie-Cecile1ORCID,Landrein Benoit1ORCID,Jaillais Yvon1ORCID

Affiliation:

1. Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, CNRS, INRAE, 69342 Lyon, France

2. LYMIC-PLATIM imaging and microscopy core facility, Univ Lyon, SFR Biosciences, ENS de Lyon, Inserm US8, CNRS UMS3444, UCBL-50 Avenue Tony Garnier, 69007 Lyon, France

Abstract

Abstract At the center of cell biology is our ability to image the cell and its various components, either in isolation or within an organism. Given its importance, biological imaging has emerged as a field of its own, which is inherently highly interdisciplinary. Indeed, biologists rely on physicists and engineers to build new microscopes and imaging techniques, chemists to develop better imaging probes, and mathematicians and computer scientists for image analysis and quantification. Live imaging collectively involves all the techniques aimed at imaging live samples. It is a rapidly evolving field, with countless new techniques, probes, and dyes being continuously developed. Some of these new methods or reagents are readily amenable to image plant samples, while others are not and require specific modifications for the plant field. Here, we review some recent advances in live imaging of plant cells. In particular, we discuss the solutions that plant biologists use to live image membrane-bound organelles, cytoskeleton components, hormones, and the mechanical properties of cells or tissues. We not only consider the imaging techniques per se, but also how the construction of new fluorescent probes and analysis pipelines are driving the field of plant cell biology.

Funder

European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme

ANR caLIPSO

ANR STAYING-TIGHT

ANR PLANTSCAPE

ANR ChromAuxi

French Ministry of Higher Education

CNRS/University of Melbourne network

ENS de Lyon and from the BAP department of INRAE

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Plant Science

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