Autologous Airway Basal Cell Transplantation Alleviates Airway Epithelium Defect in Recurrent Benign Tracheal Stenosis

Author:

Ye Yong-Shun12,Chen Di-Fei1ORCID,Liu Ming1,Luo Yu-Long13,Chen Huan-Jie1,Zeng Hai-Kang1,Liu Jing-Wei1,Zhu Yi-Ping14,Song Xin-Yu1,Lin Chang-Qin2,Su Zhu-Quan1,Li Shi-Yue1

Affiliation:

1. State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, National Clinical Research Center for Respiratory, Guangzhou Institute of Respiratory Disease , Guangzhou, Guangdong , People’s Republic of China

2. Department of Pulmonary and Critical Care Medicine, Huizhou Municipal Central Hospital , Huizhou, Guangdong , People’s Republic of China

3. Innovation Centre for Advanced Interdisciplinary Medicine, Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University , Guangzhou, Guangdong , People’s Republic of China

4. Nanshan Medicine Innovation Institute , Guangdong Province, Guangzhou, Guangdong , People’s Republic of China

Abstract

Abstract Background Airway epithelium defects are a hallmark of recurrent benign tracheal stenosis (RBTS). Reconstructing an intact airway epithelium is of great importance in airway homeostasis and epithelial wound healing and has great potential for treating tracheal stenosis. Methods An experimental study was conducted in canines to explore the therapeutic effect of autologous basal cell transplantation in restoring airway homeostasis. First, airway mucosae from human patients with recurrent tracheal stenosis were analyzed by single-cell RNA sequencing. Canines were then randomly divided into tracheal stenosis, Stent, Stent + Cells, and Stent + Cells + Biogel groups. Autologous airway basal cells of canines in the Stent + Cells and Stent + Cells + Biogel groups were transplanted onto the stenotic airway after modeling. A biogel was coated on the airway prior to basal cell transplantation in the Stent + Cells + Biogel group. After bronchoscopic treatments, canines were followed up for 16 weeks. Results Single-cell RNA sequencing demonstrated packed airway basal cells and an absence of normal airway epithelial cells in patients with RBTS. Autologous airway basal cell transplantation, together with biogel coating, was successfully performed in the canine model. Follow-up observation indicated that survival time in the Stent + Cells + Biogel group was significantly prolonged, with a higher (100%) survival rate compared with the other groups. In terms of pathological and bronchoscopic findings, canines that received autologous basal cell transplantation showed a reduction in granulation hyperplasia as well as airway re-epithelialization with functionally mature epithelial cells. Conclusions Autologous airway basal cell transplantation might serve as a novel regenerative therapy for airway re-epithelialization and inhibit recurrent granulation hyperplasia in benign tracheal stenosis.

Funder

Guangzhou Science and Technology Program key projects

Guangzhou Regenerative Medicine and Health Guangdong Laboratory

Innovation team of respiratory diseases and regenerative medicine

Zhongnanshan Medical Foundation

Basic Research Foundation of Guangzhou

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Developmental Biology,General Medicine

Reference29 articles.

1. Postintubation tracheal stenosis;Wain;Semin Thorac Cardiovasc Surg,2009

2. Laryngotracheal resection and reconstruction;Zeeshan,2014

3. Superior strategy in benign tracheal stenosis treatment: surgery or endoscopy;Aydogmus,2021

4. Epithelial repair mechanisms in the lung;Crosby,2010

5. Airway epithelial regulation of pulmonary immune homeostasis and inflammation;Hallstrand,2014

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