A single-cell transcriptome atlas of the aging human and macaque retina

Author:

Yi Wenyang1,Lu Yufeng23,Zhong Suijuan45,Zhang Mei16,Sun Le23,Dong Hao23,Wang Mengdi2,Wei Min1,Xie Haohuan1,Qu Hongqiang7,Peng Rongmei7,Hong Jing7,Yao Ziqin1,Tong Yunyun1,Wang Wei23ORCID,Ma Qiang23,Liu Zeyuan23,Ma Yuqian1,Li Shouzhen1,Yin Chonghai2,Liu Jianwei2,Ma Chao8,Wang Xiaoqun29310ORCID,Wu Qian45,Xue Tian169

Affiliation:

1. Eye Center, The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China

2. State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Brain-Intelligence Technology (Shanghai), Bioland Laboratory (Guangzhou), Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

3. University of Chinese Academy of Sciences, Beijing 100049, China

4. State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, 100875, China

5. IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, 100875, China

6. Neurodegenerative Disorder Research Center, CAS Key Laboratory of Brain Function and Disease, University of Science and Technology of China, Hefei 230026, China

7. Department of Ophthalmology, Peking University Third Hospital Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, 100191, China

8. Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China

9. Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China

10. Advanced Innovation Center for Human Brain Protection, Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China

Abstract

Abstract The human retina is a complex neural tissue that detects light and sends visual information to the brain. However, the molecular and cellular processes that underlie aging primate retina remain unclear. Here, we provide a comprehensive transcriptomic atlas based on 119,520 single cells of the foveal and peripheral retina of humans and macaques covering different ages. The molecular features of retinal cells differed between the two species, suggesting the distinct regional and species specializations of the human and macaque retinae. In addition, human retinal aging occurred in a region- and cell-type- specific manner. Aging of human retina exhibited a foveal to peripheral gradient. MYO9A− rods and a horizontal cell subtype were greatly reduced in aging retina, indicating their vulnerability to aging. Moreover, we generated a dataset showing the cell-type- and region- specific gene expression associated with 55 types of human retinal disease, which provides a foundation to understand the molecular and cellular mechanisms underlying human retinal diseases. Together, these datasets are valuable for understanding the molecular characteristics of primate retina, as well as the molecular regulation of aging progression and related diseases.

Publisher

Oxford University Press (OUP)

Subject

Multidisciplinary

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