Engineering the enzyme toolbox to tailor glycosylation in small molecule natural products and protein biologics

Author:

Ouadhi Sara12,López Dulce María Valdez12,Mohideen F Ifthiha3,Kwan David H12ORCID

Affiliation:

1. Centre for Applied Synthetic Biology, Concordia University , Montreal, QC H4B 2A6, Canada

2. PROTEO, Quebec Network for Research on Protein Function, Structure, and Engineering , Quebec City, QC G1V 0A6, Canada

3. University of Alberta Department of Chemistry, , Edmonton, AB T6G 2G2, Canada

Abstract

Abstract Many glycosylated small molecule natural products and glycoprotein biologics are important in a broad range of therapeutic and industrial applications. The sugar moieties that decorate these compounds often show a profound impact on their biological functions, thus biocatalytic methods for controlling their glycosylation are valuable. Enzymes from nature are useful tools to tailor bioproduct glycosylation but these sometimes have limitations in their catalytic efficiency, substrate specificity, regiospecificity, stereospecificity, or stability. Enzyme engineering strategies such as directed evolution or semi-rational and rational design have addressed some of the challenges presented by these limitations. In this review, we highlight some of the recent research on engineering enzymes to tailor the glycosylation of small molecule natural products (including alkaloids, terpenoids, polyketides, and peptides), as well as the glycosylation of protein biologics (including hormones, enzyme-replacement therapies, enzyme inhibitors, vaccines, and antibodies).

Funder

Natural Sciences and Engineering Research Council of Canada

Publisher

Oxford University Press (OUP)

Subject

Molecular Biology,Biochemistry,Bioengineering,Biotechnology

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Non-Native Site-Selective Enzyme Catalysis;Chemical Reviews;2023-07-31

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