Decreased Km to dNTPs is an essential M-MuLV reverse transcriptase adoption required to perform efficient cDNA synthesis in One-Step RT-PCR assay
Author:
Affiliation:
1. Thermo Fisher Scientific Baltics, UAB, LT-02241 Vilnius, Lithuania
2. JSC Diagnolita, LT-10257 Vilnius, Lithuania
Publisher
Oxford University Press (OUP)
Subject
Molecular Biology,Biochemistry,Bioengineering,Biotechnology
Link
http://academic.oup.com/peds/article-pdf/31/3/79/24506733/gzy003.pdf
Reference52 articles.
1. Human Immunodeficiency Virus Reverse Transcriptase Displays Dramatically Higher Fidelity under Physiological Magnesium Conditions In Vitro
2. HTSeq--a Python framework to work with high-throughput sequencing data
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4. Further increase in thermostability of Moloney murine leukemia virus reverse transcriptase by mutational combination
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