Determination of Nitroimidazole Metabolites in Swine and Turkey Muscle by Liquid Chromatography

Abstract

Abstract The method described can determine hydroxy dimetridazole and hydroxy ipronidazole in turkey and swine muscle tissue at levels of 2 ppb. After an initial extraction with ethyl acetate and subsequent evaporation, the samples are solvated in acid and back-extracted. A C18 solid-phase extraction is performed after neutralization of the acidic extract. The basis for quantitation was an external standard containing both analytes. After performing 10 recovery curves with blank control tissue spiked at 0, 1,2, and 4 ppb, the mean recoveries of hydroxy dimetridazole and hydroxy ipronidazole were 61.8 and 64.6%, respectively. Recovery variation was less than 20% for both analytes over all 10 curves. The performance of the extraction method was verified in tissues incurred through the dosing of live animals.