Identification of Nile Perch (Lates niloticus), Grouper (Epinephelus guaza), and Wreck Fish (Polyprion americanus) Fillets by PCR Amplification of the 5S rDNA Gene-Reference-Cited by-同舟云学术

Identification of Nile Perch (Lates niloticus), Grouper (Epinephelus guaza), and Wreck Fish (Polyprion americanus) Fillets by PCR Amplification of the 5S rDNA Gene

Published:2001-05-01 Issue:3 Volume:84 Page:777-781
ISSN:1060-3271
Container-title:Journal of AOAC INTERNATIONAL
language:en
Short-container-title:

Author:

Asensio Luis¹,González Isabel¹,Fernández Alicia¹,Céspedes Ana¹,Rodríguez Miguel A¹,Hernández Pablo E¹,García Teresa¹

Affiliation:

1. Universidad Complutense, Facultad de Veterinaria, Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), 28040 Madrid, Spain

Abstract

Abstract Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) were differentiated by polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene. The design of 3 species-specific primers complementary to the nontranscribed intergenic spacer region from the 5S rDNA molecule allowed amplification of clearly distinguishable gene fragments in each fish species. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

Link

http://academic.oup.com/jaoac/article-pdf/84/3/777/32415855/jaoac0777.pdf

Cited by 24 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Development of 5S rDNA-Based Molecular Markers for the Identification of Populus deltoides Bartr. ex Marshall, Populus nigra L., and Their Hybrids;Forests;2018-09-29

2. Seafood Authentication using Foodomics;Genomics, Proteomics and Metabolomics in Nutraceuticals and Functional Foods;2015-08-14

3. Species Identification of Seafood;The Seafood Industry;2012-02-20

4. Molecular differentiation of the species of two squid families (Loliginidae and Ommastrephidae) based on a PCR study of the 5S rDNA gene;Food Control;2011-01

5. Polymerase chain reaction banding patterns of the 5S rDNA gene as a diagnostic tool for the discrimination of South American mullets of the genus Mugil;Aquaculture Research;2010-11-18