Collaborative exercise: analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform-Reference-Cited by-同舟云学术

Collaborative exercise: analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform

Published:2024-01-05 Issue:1 Volume:9 Page:
ISSN:2096-1790
Container-title:Forensic Sciences Research
language:en
Short-container-title:

Author:

Kampmann Marie-Louise¹²,Fleckhaus Jan³⁴,Børsting Claus¹²,Jurtikova Helena⁵⁶,Piters Alice⁷,Papin Julien⁷,Gauthier Quentin⁸,Ghemrawi Mirna⁸,Doutremepuich Christian⁷,McCord Bruce⁸,Schneider Peter M³⁴,Drabek Jiri⁵⁶,Morling Niels¹²

Affiliation:

1. Section of Forensic Genetics , Department of Forensic Medicine, Faculty of Health and Medical Sciences, Copenhagen , Denmark

2. University of Copenhagen , Department of Forensic Medicine, Faculty of Health and Medical Sciences, Copenhagen , Denmark

3. Institute of Legal Medicine , Faculty of Medicine and University Clinic, , Cologne , Germany

4. University of Cologne , Faculty of Medicine and University Clinic, , Cologne , Germany

5. Institute of Molecular and Translational Medicine , Faculty of Medicine and Dentistry, , Olomouc , the Czech Republic

6. Palacky University Olomouc and the University Hospital Olomouc , Faculty of Medicine and Dentistry, , Olomouc , the Czech Republic

7. Laboratoire d’Hématologie Médico-Légale , Bordeaux Cedex , France

8. Department of Chemistry and Biochemistry, Florida International University , Miami, FL , USA

Abstract

Abstract Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator. Several molecular biomarkers are available for the estimation of chronological age, and currently, DNA methylation patterns are the most promising. In this study, a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories. The assay comprised bisulfite treatment of the extracted DNA, amplification of five CpG loci (in the genes of ELOVL2, C1orf132, TRIM59, KLF14, and FHL2), and sequencing of the amplicons using the PyroMark Q48 platform. Blood samples from 49 individuals with ages ranging from 18 to 64 years as well as negative and methylation controls were analyzed. An existing age estimation model was applied to display a mean absolute deviation of 3.62 years within the reference data set. Key points

Funder

Palacky University

National Institute of Justice

Department of Justice

Department of Chemistry and Biochemistry

Florida International University

Publisher

Oxford University Press (OUP)

Link

https://academic.oup.com/fsr/advance-article-pdf/doi/10.1093/fsr/owad055/55118794/owad055.pdf

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