Challenge Inoculum for Hepatitis C Virus Controlled Human Infection Model

Author:

Liang T Jake1ORCID,Law John L M2,Pietschmann Thomas3,Ray Stuart C4,Bukh Jens5,Bull Rowena6,Chung Raymond T7,Tyrrell D Lorne2,Houghton Michael2,Rice Charles M8

Affiliation:

1. Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health , Bethesda, Maryland , USA

2. Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, University of Alberta , Edmonton , Canada

3. Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research , Hannover , Germany

4. Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine , Baltimore, Maryland , USA

5. Copenhagen Hepatitis C Program (CO-HEP), Department of Infectious Diseases, Copenhagen University Hospital; Hvidovre and Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen , Denmark

6. Liver Center, Massachusetts General Hospital and Harvard Medical School , Boston, Massachusetts , USA

7. School of Biomedical Sciences and The Kirby Institute, Medicine and Health, University of New South Wales , Sydney , Australia

8. Laboratory of Virology and Infectious Disease, The Rockefeller University , New York, New York , USA

Abstract

Abstract For any controlled human infection model (CHIM), a safe, standardized, and biologically relevant challenge inoculum is necessary. For hepatitis C virus (HCV) CHIM, we propose that human-derived high-titer inocula of several viral genotypes with extensive virologic, serologic, and molecular characterizations should be the most appropriate approach. These inocula should first be tested in human volunteers in a step-wise manner to ensure safety, reproducibility, and curability prior to using them for testing the efficacy of candidate vaccines.

Funder

Toronto General Research Institute

National Institutes of Health

Johns Hopkins University

Canadian Institutes of Health Research

Canadian Network on Hepatitis C

Public Health Agency of Canada

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Microbiology (medical)

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