Affiliation:
1. Rostock University Medical Center, Institute for Biostatistics and Informatics in Medicine and Ageing Research , Rostock , Germany
2. Humboldt-University of Berlin, Institute of Biology , Berlin , Germany
3. Budapest University of Technology and Economics, Department of Measurement and Information Systems , Budapest , Hungary
4. Rostock University Medical Center, Institute for Clinical Chemistry and Laboratory Medicine , Rostock , Germany
Abstract
Abstract
Health(span)-related gene clusters/modules were recently identified based on knowledge about the cross-species genetic basis of health, to interpret transcriptomic datasets describing health-related interventions. However, the cross-species comparison of health-related observations reveals a lot of heterogeneity, not least due to widely varying health(span) definitions and study designs, posing a challenge for the exploration of conserved healthspan modules and, specifically, their transfer across species. To improve the identification and exploration of conserved/transferable healthspan modules, here we apply an established workflow based on gene co-expression network analyses employing GEO/ArrayExpress data for human and animal models, and perform a comprehensive meta-study of the resulting modules related to health(span), yielding a small set of literature backed health(span) candidate genes. For each experiment, WGCNA (weighted gene correlation network analysis) was used to infer modules of genes which correlate in their expression with a ‘health phenotype score’ and to determine the most-connected (hub) genes (and their interactions) for each such module. After mapping these hub genes to their human orthologs, 12 health(span) genes were identified in at least two species (ACTN3, ANK1, MRPL18, MYL1, PAXIP1, PPP1CA, SCN3B, SDCBP, SKIV2L, TUBG1, TYROBP, WIPF1), for which enrichment analysis by g:profiler found an association with actin filament-based movement and associated organelles, as well as muscular structures. We conclude that a meta-study of hub genes from co-expression network analyses for the complex phenotype health(span), across multiple species, can yield molecular-mechanistic insights and can direct experimentalists to further investigate the contribution of individual genes and their interactions to health(span).
Funder
European Union's Horizon 2020 research and innovation programme
NRDI Office
Artificial Intelligence National Laboratory Programme
BMBF
Hungarian Academy of Sciences
European Union
Publisher
Oxford University Press (OUP)
Subject
Applied Mathematics,Computer Science Applications,Genetics,Molecular Biology,Structural Biology
Cited by
1 articles.
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