Sustainable production of single-cell oil and protein from wastepaper hydrolysate: identification and optimization of a Rhodotorula mucilaginosa strain as a promising yeast

Author:

Campos-Valdez Amador1,Kirchmayr Manuel R1,Barrera-Martínez Iliana2,Casas-Godoy Leticia2ORCID

Affiliation:

1. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Biotecnología Industrial , Camino Arenero 1227 , El Bajío, 45019 Zapopan, Jalisco, México

2. CONAHCYT – Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Biotecnología Industrial , Camino Arenero 1227 , El Bajío, 45019 Zapopan, Jalisco, México

Abstract

Abstract This study investigated the potential of wastepaper hydrolysate as a sustainable and low-cost carbon source for single-cell oil and protein production, attending to the growing need for alternative feedstocks and waste management strategies. Wastepaper, characterized by its high carbohydrate content, was subjected to enzymatic and chemo-enzymatic treatments for carbohydrate release. The chemo-enzymatic treatment performed better, yielding 65.3 g l−1 of fermentable sugars. A total of 62 yeast strains were screened for single-cell oil accumulation, identifying Rhodotorula mucilaginosa M1K4 as the most advantageous oleaginous yeast. M1K4 lipid production was optimized in liquid culture, and its fatty acid profile was analyzed, showing a high content of industrially valuable fatty acids, particularly palmitic (28%) and oleic (51%). Batch-culture of M1K4 in a 3-l reactor demonstrated the strain’s ability to utilize wastepaper hydrolysate as a carbon source, with dry cell weight, total lipid and protein production of 17.7 g l−1, 4.5 g l−1, and 2.1 g l−1, respectively. Wastepaper as a substrate provides a sustainable solution for waste management and bioproduction. This research highlights the potential of R. mucilaginosa for lipid and protein production from wastepaper hydrolysate.

Funder

Consejo Estatal de Ciencia y Tecnología de Jalisco

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Microbiology

Reference51 articles.

1. Waste paper to bioethanol: current and future prospective;Al-Azkawi;Biofuels Bioprod Biorefining,2019

2. Screening of new oleaginous yeasts for single cell oil production, hydrolytic potential exploitation and agro-industrial by-products valorization;Ayadi;Inst Chem Eng,2018

3. Utilization of wheat bran acid hydrolysate by Rhodotorula mucilaginosa Y-MG1 for microbial lipid production as feedstock for biodiesel synthesis;Ayadi;Biomed Res Int,2019

4. Yarrowia lipolytica as a model for bio-oil production;Beopoulos;Prog Lipid Res,2009

5. Chapter 1–global climate change and its impact on agriculture;Bhattacharya,2019

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3