Riboflavin overproduction on lignocellulose hydrolysate by the engineered yeast Candida famata

Author:

Dzanaeva Ljubov S1ORCID,Wojdyła Dominik2ORCID,Fedorovych Dariya V1ORCID,Ruchala Justyna2ORCID,Dmytruk Kostyantyn V1ORCID,Sibirny Andriy A12ORCID

Affiliation:

1. Institute of Cell Biology, National Academy of Sciences of Ukraine , Drahomanov St., 14/16, 79005 Lviv , Ukraine

2. Institute of Biotechnology, University of Rzeszow , Zelwerowicza 4, 35-601 Rzeszow , Poland

Abstract

Abstract Lignocellulose (dry plant biomass) is an abundant cheap inedible residue of agriculture and wood industry with great potential as a feedstock for biotechnological processes. Lignocellulosic substrates can serve as valuable resources in fermentation processes, allowing the production of a wide array of chemicals, fuels, and food additives. The main obstacle for cost-effective conversion of lignocellulosic hydrolysates to target products is poor metabolism of the major pentoses, xylose and L-arabinose, which are the second and third most abundant sugars of lignocellulose after glucose. We study the oversynthesis of riboflavin in the flavinogenic yeast Candida famata and found that all major lignocellulosic sugars, including xylose and L-arabinose, support robust growth and riboflavin synthesis in the available strains of C. famata. To further increase riboflavin production from xylose and lignocellulose hydrolysate, genes XYL1 and XYL2 coding for xylose reductase and xylitol dehydrogenase were overexpressed. The resulting strains exhibited increased riboflavin production in both shake flasks and bioreactors using diluted hydrolysate, reaching 1.5 g L−1.

Funder

National Science Centre of Poland

Simons Foundation

NCN

Publisher

Oxford University Press (OUP)

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