Characterization of the surugamide biosynthetic gene cluster of TUA-NKU25, a<i>Streptomyces diastaticus</i>strain isolated from Kusaya, and its effects on salt-dependent growth-Reference-Cited by-同舟云学术

Characterization of the surugamide biosynthetic gene cluster of TUA-NKU25, aStreptomyces diastaticusstrain isolated from Kusaya, and its effects on salt-dependent growth

Published:2022-12-10 Issue:3 Volume:87 Page:320-329
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Takeuchi Aoi¹,Hirata Asahi²,Teshima Aiko²,Ueki Miu¹,Satoh Takumi³,Matsuda Kenichi⁴^ORCID,Wakimoto Toshiyuki⁴^ORCID,Arakawa Kenji²^ORCID,Ishikawa Morio¹,Suzuki Toshihiro¹^ORCID

Affiliation:

1. Department of Fermentation Sciences, Faculty of Applied Biosciences, Tokyo University of Agriculture , 1-1-1 Sakuragaoka, Setagaya, Tokyo , Japan

2. Graduate School of Integrated Sciences for Life, Hiroshima University , 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima , Japan

3. Department of Molecular Microbiology, Faculty of Life Sciences, Tokyo University of Agriculture , 1-1-1 Sakuragaoka, Setagaya, Tokyo , Japan

4. Faculty of Pharmaceutical Sciences, Hokkaido University , Kita 12, Nishi 6, Kita-ku, Sapporo, Hokkaido , Japan

Abstract

ABSTRACTKusaya, a traditional Japanese fermented fish product, is known for its high preservability, as it contains natural antibiotics derived from microorganisms, and therefore molds and yeasts do not colonize it easily. In this study, the Streptomyces diastaticus strain TUA-NKU25 was isolated from Kusaya, and its growth as well as the production of antibiotics were investigated. Strain TUA-NKU25 showed advantageous growth characteristics in the presence, but not in the absence, of sodium chloride (NaCl). Antimicrobial assay, high-performance liquid chromatography, and electrospray ionization-mass spectrometry analysis showed that this strain produced surugamide A and uncharacterized antimicrobial compound(s) during growth in the presence of NaCl, suggesting that the biosynthesis of these compounds was upregulated by NaCl. Draft genomic analysis revealed that strain TUA-NKU25 possesses a surugamide biosynthetic gene cluster (sur BGC), although it is incomplete, lacking surB/surC. Phylogenetic analysis of strain TUA-NKU25 and surugamide-producing Streptomyces showed that sur BGC formed a clade distinct from other known groups.

Funder

Natural Science Center for Basic Research and Development

Hiroshima University

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbac201/48497601/zbac201.pdf

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