Transport engineering using tobacco transporter NtJAT1 enhances alkaloid production in <i>Escherichia coli</i>-Reference-Cited by-同舟云学术

Transport engineering using tobacco transporter NtJAT1 enhances alkaloid production in Escherichia coli

Published:2022-04-15 Issue: Volume: Page:
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Yamada Yasuyuki¹^ORCID,Nakagawa Akira²,Sato Fumihiko³⁴^ORCID,Minami Hiromichi²,Shitan Nobukazu¹^ORCID

Affiliation:

1. Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University, Motoyamakita-machi, Higashinada-ku, Kobe, 658-8558, Japan

2. Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi-machi, Ishikawa, 921-8836, Japan

3. Department of Plant Gene and Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan

4. Graduate School of Science, Osaka Prefecture University, Sakai, 599-8531, Japan

Abstract

ABSTRACT Transporters have been used in the production of plant metabolites in microorganisms. This study introduced a tobacco multidrug and toxic compound extrusion transporter, NtJAT1, into alkaloid-producing Escherichia coli cells. NtJAT1 expression enhanced alkaloid production secretion into the medium by 14-fold. Our findings further demonstrate the usefulness of the transport engineering approach.

Publisher

Informa UK Limited

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbac056/43383910/zbac056.pdf

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