Quantitative proteomic analysis uncovers protein-expression profiles during gonadotropin-dependent folliculogenesis in mice

Author:

Zhang Shuo1,Mu Lu1,Wang Haoran1,Xu Xueqiang1,Jia Longzhong1,Niu Shudong1,Wang Yibo1,Wang Peike1,Li Lingyu1,Chai Junyi1,Li Zhen2,Zhang Yan1,Zhang Hua1

Affiliation:

1. China Agricultural University State Key Laboratory of Agrobiotechnology, College of Biological Sciences, , Beijing, China

2. China Agricultural University State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, , Beijing, China

Abstract

AbstractOvarian follicle is the basic functional unit of female reproduction, and is composed of oocyte and surrounding granulosa cells. In mammals, folliculogenesis strictly rely on gonadotropin regulations to determine the ovulation and the quality of eggs. However, the dynamic changes of protein-expressing profiles in follicles at different developmental stages remain largely unknown. By performing mass-spectrometry-based quantitative proteomic analysis of mouse follicles, we provide a proteomic database (~3000 proteins) that covers three key stages of gonadotropin-dependent folliculogenesis. By combining bioinformatics analysis with in situ expression validation, we showed that our proteomic data well reflected physiological changes during folliculogenesis, which provided potential to predict unknown regulators of folliculogenesis. Additionally, by using the oocyte structural protein zona pellucida protein 2 as the internal control, we showed the possibility of our database to predict the expression dynamics of oocyte-expressing proteins during folliculogenesis. Taken together, we provide a high-coverage proteomic database to study protein-expression dynamics during gonadotropin-dependent folliculogenesis in mammals.

Funder

Chinese Universities Scientific Fund

National Key Research and Development Program of China

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,General Medicine,Reproductive Medicine

Reference87 articles.

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1. The Ovarian Cycle (Estrous Cycle) and Follicular Growth;Bovine Maternal Support and Embryo Survival;2024

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