Affiliation:
1. From the Boston Medical Laboratory, Inc., 15 Lunda St., Waltham, Mass. 02154
Abstract
Abstract
Serum folate determination by this method requires incubation of 0.1 ml of serum (or standards) with nondialyzed milk and then with tritium-labeled folate. A serum blank corrects for nonspecific binders present in the serum. Unbound folate is removed with hemoglobin-coated charcoal. The standard deviation of the results is ±0.2 µg/liter, and more than 90% of folate added to the serum is accounted for analytically. Serum folate concentrations as determined by this method agree with those obtained by microbiological assay (Lactobacillus casei), and our method is faster, simpler, and more accurate than other radioassay methods
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
10 articles.
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