International collaborative study to evaluate a recombinant L ferritin preparation as an International Standard-Reference-Cited by-同舟云学术

International collaborative study to evaluate a recombinant L ferritin preparation as an International Standard

Published:1997-09-01 Issue:9 Volume:43 Page:1582-1587
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Thorpe Susan J,Walker Dawn,Arosio Paolo¹,Heath Alan,Cook James D²,Worwood Mark³

Affiliation:

1. University of Brescia, Brescia, Italy

2. University of Kansas Medical Center, Kansas City, KS, USA

3. University of Wales College of Medicine, Cardiff, UK

Abstract

AbstractA recombinant L ferritin preparation, lyophilized in ampoules and designated 94/572, was evaluated by 18 laboratories in 9 countries for its suitability as an International Standard (IS). The preparation was assayed in a wide range of in-house and commercial immunoassays against the 2nd IS for ferritin (of spleen origin; 80/578). The immunological reactivity of the recombinant material was similar to that of the 2nd IS for ferritin in the majority of assays and demonstrated adequate stability in accelerated degradation studies. On the basis of the results presented here, the WHO Expert Committee on Biological Standardization established 94/572 as the 3rd IS for ferritin, recombinant.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/43/9/1582/32718181/clinchem1582.pdf

Reference16 articles.

1. Evidence of H- and L-chains have co-operative roles in the iron-uptake mechanism of human ferritin

2. Production and characterization of recombinant heteropolymers of human ferritin H and L chains

3. Construction of a ferroxidase center in human ferritin L-chain.

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