Estimation of Total Cholesterol in Serum by a Micro Method

Author:

Carpenter K J1,Gotsis A1,Hegsted D M1

Affiliation:

1. Department of Nutrition, Harvard School of Public Health, Boston, Mass

Abstract

Abstract A method has been described for the estimation of total cholesterol in 0.02ml. of serum without the need for micro apparatus. It involves the measurement of the fluorescence developed after the addition of sulfuric acid to a solution of cholesterol in a mixture of 1,1,2-trichloroethane and acetic anhydride(1). The results obtained with both human and rat sera have been in good agreement with those obtained with a standard colorimetric method(2), and the standard deviation for an estimation carried through in duplicate, owing to error within the method, has been ±4-5 mg. per 100 ml. under routine conditions.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

Cited by 49 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Kenneth John Carpenter, Ph.D. (1923–2016);The Journal of Nutrition;2018-02-01

2. Determination of cholesterol in blood. Part 1;Review Journal of Chemistry;2016-01

3. Determination and Microscopic Localization of Cholesterol;Methods of Biochemical Analysis;2006-10-31

4. Iron overload can induce mild copper deficiency;Journal of Trace Elements in Medicine and Biology;2001-04

5. Copper deficiency halves serum dehydroepiandrosterone in rats;Journal of Trace Elements in Medicine and Biology;2000-10

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