Evaluation of a Cellulose-Acetate Electrophoresis System for Serum Protein Fractionation

Author:

Kaplan Alex1,Savory John2

Affiliation:

1. University Hospital and Department of Biochemistry, University of Washington, Seattle, Wash. 98105

2. postdoctoral trainee supported by Training Grant in Clinical Chemistry, T1 GM 776-01, from the National Institutes of Health

Abstract

Abstract A rapid system for the quantitative fractionation of serum proteins by electrophoresis on cellulose-acetate membranes was evaluated and found to be quite precise. The reproducibility (coefficient of variation) of the routine fractionation of a control serum carried out 40 times during a 15-week period was 2.4% for albumin and 14.2, 6.0, 6.1, and 5.2%, respectively, for the α1-, α2-, β-, and γ-globulin fractions. Normal values are given for serum protein fractions (specimens from nonprofessional blood donors) obtained by cellulose acetate electrophoresis.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

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