"U" Protein and Migration Ratios in Paper Electrophoresis

Abstract

Abstract Paper electrophoresis of serum from children with liver and kidney disease has led to recognition of a protein which migrates between the 2 and globulins. This protein has been observed by other workers, but because of confusion in nomenclature, a new designation, "U" protein, has been employed. The use of migration ratios in locating peaks of the 2 "U" protein, and globulin when these components resolve poorly has been discussed. Characterization of "U" protein by employing the periodic acid-reduced fuchsin test for carbohydrate-protein complexes and Sudan black B for lipoprotein showed: (1)"U" protein varies in its content of protein-carbohydrate complex, and (2)"U" protein may be lipo-protein. Evidence based on benzidine tests and intentional hemolysis indicated that "U" protein was not an artifact resulting from accidental hemolysis, but a protein which is found in serum under certain physiological conditions.