Use of Tris (Hydroxymethyl) Aminomethane Buffer in Moving-boundary Electrophoresis of Serum

Abstract

Abstract A tris-borate buffer solution of µ = 0.1 and pH 8.9 is shown to be satisfactory for use in the electrophoresis of human and bovine sera by the moving-boundary technic. Comparison of the patterns obtained by the use of tris-borate and barbital buffer is presented. Similar numbers of electrophoretically distinct components are found by the use of either buffer. Although the calculated mobility of albumin is slightly lower in tris-borate than in barbital, mobilities of the other serum components are similar in the two buffers. Several practical advantages of the tris-borate over the barbital mixture make the former buffer more than satisfactory as a replacement of the latter.