Affiliation:
1. Department of Biochemistry and the Biological Sciences Research Center, Child Development Institute, School of Medicine, University of North Carolina, Chapel Hill, N. C. 27514
Abstract
Abstract
We describe a sensitive automated fluorometric method for glucose in whole blood spotted on filter paper. By action of glucose oxidase (EC 1.1.3.4) on glucose, hydrogen peroxide is produced, which then reacts with 4-hydroxy-3-methoxy-phenylacetic acid (homovanillic acid) in the presence of horseradish peroxidase (EC 1.11.1.7) to form a fluorescent condensation product. Obtained at a rate of 60 samples per hour, the results are accurate, linear, and reproducible. Whole blood glucose can be determined by this method with an error (CV) of less than 5%.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
6 articles.
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