Affiliation:
1. Columbia Presbyterian Medical Center, Babies’ Hospital, Clinical Chemistry Laboratory, 3975 Broadway, New York, N. Y. 10032
Abstract
Abstract
We developed a new micro-scale procedure for determination of protein in cerebrospinal fluid and urine. The sample (50 µl) is mixed with a trichloroacetic acid—Ponceau S dye solution. The proteins, together with a proportional amount of dye, are precipitated and the red precipitate is dissolved in dilute sodium hydroxide solution, giving a violet-colored solution. This color, linearly proportional to the amount of protein present (up to 150 mg/dl), is measured spectrophotometrically at 560 nm. By varying the sample size alone, protein concentrations up to 1500 mg/dl can be measured. The detection limit for the method is 2 mg/dl of sample. Precipitation of the proteins is independent of temperature and albuminbinding compounds, and is not appreciably affected by the albumin—gamma globulin ratio.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
189 articles.
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