Multicenter evaluation of the Paragon CZETM 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing

Author:

Bienvenu Jacques1,Graziani Maria Stella2,Arpin François1,Bernon Hélène1,Blessum Cynthia3,Marchetti Cristina2,Righetti Gabriella2,Somenzini Manuela4,Verga Gioacchino5,Aguzzi Francesco4

Affiliation:

1. Laboratoire d’Immunologie, Centre Hospitalier Lyon Sud, 69495 Pierre-Bénite, France

2. Laboratorio di Chimica Clinica ed Ematologia, Ospedale Civile Maggiore 37126 Verona, Italy

3. Beckman Instruments Inc., 200 S. Kraemer Blvd., Brea, CA 92621

4. Laboratorio Analisi, Ospedale di Stradella, 27049 Stradella, Italy

5. Beckman Analytical SPA, Via Roma, 108, 20060 Cassina De Pecchi, Italy

Abstract

Abstract Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZETM 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were <2% for albumin and γ-globulins and 4–7% for α1-, α2-, and β-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was <0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations >10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

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