Affiliation:
1. Center for Population Studies, Harvard University, 665 Huntington Ave., Boston, Mass. 02115
Abstract
Abstract
A method is reported for determining urinary xanthenuric acid (XA), a metabolite of tryptophan. XA is extracted from urine with isobutanol (2-methyl-1-propanol), transferred to ammonium hydroxide, and separated from other urinary metabolites on thin-layer cellulose plates with use of 0.2 acetate buffer, mol/liter, pH 5.4. The XA is measured spectrophotometrically after diazotization with 2-chloro-4-nitroaniline. The method, which is specific for XA, can be used to measure amounts as low as 0.4 µg/ml of urine. It has a range from 0 to 24 µg/ml, and results within this range are reproducible within approximately ±5%. Data obtained by this procedure are comparable to results reported for other methods.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
4 articles.
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