Affiliation:
1. Department of Pathology, The Medical College of Wisconsin, 8700 West Wisconsin Ave., Milwaukee, Wis. 53226
Abstract
Abstract
Because of the inaccuracy of bilirubin (BIL) determinations, we have investigated some sources of error associated with the most commonly used methods. Inadequate standardization appears to be the most common error. BIL standards in either human serum albumin (HSA), bovine serum albumin (BSA), or pooled human serum were analyzed by these methods. Molar absorptivities (ε) of azobilirubin by the Jendrassik—Gróf procedure were practically identical in all three protein bases. The Meites—Hogg and Malloy—Evelyn methods gave substantially higher ε values with BIL in serum than with BIL in either HSA or BSA. The precision of all methods was good, but best with the Jendrassik—Gróf procedure. BIL standards can be prepared with good reproducibility (CV <0.5%). Standards deteriorate appreciably at -23 °C but are stable at -70 °C. Our data indicate a need for improved commercial BIL controls.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
149 articles.
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