Affiliation:
1. Department of Pediatrics, University of Oregon Medical School, Portland, Ore. 97201
Abstract
Abstract
A simple micro (0.1 ml.) colorimetric method is described for the determination of serum calcium. This method employs the differential chelating characteristic of glyoxyl bis (2-hydroxyanil) in the presence of other divalent cations. The serum sample is placed in a colorimetric tube followed by additions of water, glyoxyl bis (2-hydroxyanil) reagent, and alkaline carbonate color developer. Chloroform is added to extract the chromogen, and its transmittance at 535 mµ is determined.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
6 articles.
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