Enhancing Effect of Surfactant and Protein on Hydrolysis of Thymolphthalein Monophosphate by Purified Prostatic Acid Phosphatase-Reference-Cited by-同舟云学术

Enhancing Effect of Surfactant and Protein on Hydrolysis of Thymolphthalein Monophosphate by Purified Prostatic Acid Phosphatase

Published:1975-11-01 Issue:12 Volume:21 Page:1761-1765
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Foti Andras G¹,Herschman Harvey²,Cooper J Fenimore³,imFeld Hedi¹

Affiliation:

1. Department of Research, Southern California Permanente Medical Group, Los Angeles, Calif. 90027

2. Department of Biological Chemistry and Laboratory of Nuclear Medicine, UCLA School of Medicine, Los Angeles, Calif. 90024

3. Department of Urology, Southern California Permanente Medical Group and Kaiser Foundation Hospital, Los Angeles, Calif. 90027

Abstract

Abstract Purified prostatic acid phosphatase catalyzes the hydrolysis of thymolphthalein monophosphate 10-fold faster if an optimal concentration of Brij 35 (a wetting agent) or protein (bovine serum albumin or human serum proteins) is present. Results of gel filtration, dialysis, and sucrose density-gradient centrifugation analysis suggest that the substrate must combine with detergent or protein before the enzyme can catalyze its hydrolysis.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/21/12/1761/32807445/clinchem1761.pdf

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