Affiliation:
1. Clinical Biochemistry Section, Department of Pathology, Sepoy Lines, Singapore-3
Abstract
Abstract
A simple method for the determination of ascorbic acid in urine was developed. The method is based on the quantitative reduction of mercuric chloride to mercurous chloride at pH 3.5-5.0. The insoluble mercurous chloride was separated by centrifugation, dissolved in excess standard iodine-potassium iodide solution and back-titrated with standard sodium thiosulphate. The method has precision, and as little as 1.0 mg. of ascorbic acid per 100 ml. of urine can be determined with an accuracy of ± 3%. Common substances found in urine do not interfere.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
4 articles.
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