Affiliation:
1. Roche Institute of Molecular Biology, Nutley, N.J. 07110
Abstract
Abstract
We describe a simple, rapid, and sensitive procedure for measuring dopamine-β-hydroxylase activity in human blood. The assay is based on the enzymatic conversion of tyramine to octopamine [1-(p-hydroxyphenyl)-2-aminoethanol], which is then oxidized to p-hydroxybenzaldehyde and determined photometrically. Activities are assayed on 2-20 µl of human serum or plasma obtained from fingertip blood samples. The method should prove useful in clinical studies, including the field of pediatrics.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
398 articles.
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