Analytic Methods for Bilirubin in Blood Plasma-Reference-Cited by-同舟云学术

Analytic Methods for Bilirubin in Blood Plasma

Published:1961-12-01 Issue:6 Volume:7 Page:603-625
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Watson Derek¹

Affiliation:

1. Department of Biochemistry, The Royal Women's Hospital, Melbourne, N.3, Australia

Abstract

Abstract A critical evaluation of existing colorimetric and spectrophotometric methods for the determination of bilirubin in blood plasma is presented. The stoichiometric equivalences of the reagents used in the diazo coupling reaction are re-evaluated. The elimination of turbidity is discussed, as well as methods using diazo-coupling promoters. Recommendations are made concerning the preservation of specimens to prevent oxidation and procedures that give maximal specificity for the type of bilirubin pigment being assayed.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/7/6/603/32873741/clinchem0603.pdf

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