Fully automated liquid-chromatographic determination of amino acids.

Abstract

Abstract This inexpensive method for fully automated amino acid analysis combines the advantages of automated precolumn derivatization with o-phthaldialdehyde and favorable analytical conditions to separate and quantify 30 amino acids found in normal plasma. The system can run unattended for almost four days, during which the data are processed automatically by a personal computer and a maximum of 76 samples and 19 standards can be processed (cycle time per analysis: 55 min). Only 1 microL of deproteinized plasma is required per analysis. Coefficients of variation for retention times and areas measured for all relevant amino acids are less than 1% and 3%, respectively. The system described is well suited for quick, sensitive operation in daily practice.