Paper-Chromatographic Quantitation of 4-Hydroxy-3-methoxymandelic Acid (VMA) in Urine-Reference-Cited by-同舟云学术

Paper-Chromatographic Quantitation of 4-Hydroxy-3-methoxymandelic Acid (VMA) in Urine

Published:1971-09-01 Issue:9 Volume:17 Page:903-907
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Vahidi Ardeshir¹,Roberts Henry R¹,San Filippo Joseph¹,Sankar D V Siva¹

Affiliation:

1. Squibb Institute for Medical Research, New Brunswick, N. J. 08903 (A. V. and H. R. R.); and the Department of Chemistry, Long Island University, New York and Biochemical Research Laboratory, Creedmoor Children’s Hospital, Queens Village, N. Y. 11427 (J. S. F. and D. V. S. S.)

Abstract

Abstract We describe a quantitative paper-chromatographic method for assaying 3-methoxy-4-hydroxymandelic acid (VMA) in urine. VMA is extracted from acidified urine into ethyl acetate, separated from interfering amine, acid, and alcoholic metabolites by paper chromatography, and reacted with diazotized p-nitroaniline. The colored derivative is eluted and spectrophotometrically analyzed. Recoveries of replicates of standard and sample solutions on the same chromatogram exceeded 95%.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/17/9/903/32820661/clinchem0903.pdf

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1. Biogenic amines and their metabolites in body fluids of normal, psychiatric and neurological subjects;Journal of Chromatography A;1989-01

2. High-performance liquid chromatographic procedure for the analysis of urinary 3-methoxy-4-hydroxymandelic acid;Journal of Chromatography B: Biomedical Sciences and Applications;1983-01

3. A Comparative Study of Five Different Methods for the Determination of 3-Methoxy-4-hydroxymandelic Acid in Urine;Clinical Chemistry and Laboratory Medicine;1983

4. Literaturhinweise für weitere Bestimmungsmethoden;Mikrochemische Methoden für neurobiologische Untersuchungen;1980

5. Separation of urinary aromatic acids and measurement of vanilmandelic acid by high-performance liquid chromatography;Chromatographia;1978-05