Affiliation:
1. Department of Chemistry, University of Maryland, College Park, Md. 20742
Abstract
Abstract
Conditions are described for coulometric titration of reduced glutathione with silver(I), in an ammoniacal medium. pH and buffer capacity of the medium are important variables. Metaphosphoric acid, 50 g/liter, is superior to metaphosphoric acid, 10 g/liter, or sulfosalicylic acid, 25 g/liter, for precipitating blood proteins. Results of the coulometric method are about the same as those obtained with spectrophotometric procedures in which alloxan or 5,5'-dithio-bis-(2-nitrobenzoic acid) is used.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
8 articles.
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