Design, Construction, and Two Applications for an Automated Flow—Cell Polarization Fluorometer with Digital Read Out: Enzyme-Inhibitor (Antitrypsin) Assay and Antigen—Antibody (Insulin—Insulin Antiserum) Assay

Abstract

Abstract We have constructed and automated a flow cell polarization fluorometer and demonstrated two specific clinical applications of fluorescence polarization assay, i.e., the enzyme-inhibitor assay of antitrypsin in serum and the antigen-antibody assay of insulin and its antibody. A signal is displayed directly and is immediately available for chart recording and (or) digital data processing. Since fluorescence polarization offers a number of choices in assay parameters and the use of different fluorescent probes, its development for rapid simultaneous measurements of multiple components in body fluids should be possible.