Affiliation:
1. Biophysics Research Laboratory, Division of Medical Biology of the Department of Medicine, Harvard Medical School and Peter Bent Brigham Hospital, Boston, Mass
Abstract
Abstract
The spectrophotometric and colorimetric procedures for the assay of serum glutamic-oxaloacetic transaminase (SGOT) activity are assessed. Directions are given for the preparation of reagents and for a procedure that corrects the errors in the spectrophotometric method without altering its basic simplicity. The colorimetric procedure employing dinitrophenylhydrazine showed poor accuracy due, in part, to a large and variable blank.
The proposed procedure eliminates the troublesome group of analytic results, "borderline" measurements, a source of diagnostic uncertainty.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
65 articles.
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