Rapid Enzymatic Measurement of Blood Lactate and Pyruvate-Reference-Cited by-同舟云学术

Rapid Enzymatic Measurement of Blood Lactate and Pyruvate

Published:1967-04-01 Issue:4 Volume:13 Page:314-325
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Marbach Edward P¹,Weil Max Harry¹

Affiliation:

1. Shock Research Unit and Department of Medicine, University of Southern California School of Medicine, and the Clinical Laboratory of the Los Angeles County General Hospital, Los Angeles, Calif. 90033

Abstract

Abstract A modification of enzymatic technics for measurement of concentrations of lactate and pyruvate in blood is described. A common protein-free filtrate is prepared by adding blood directly to cold 5% metaphosphoric acid. Theoretical yields are obtained. Recoveries for lactate range from 98 to 102% and for pyruvate from 97 to 104%. Assay of both lactate and pyruvate is simplified and has a high order of reliability. Since both analyses are completed within a period of 20 mm., the method is especially valuable in the emergent clinical situation in which measurements of lactate and pyruvate are obtained for assessment of the severity of shock and other forms of cardiorespiratory failure.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/13/4/314/32818440/clinchem0314.pdf

Cited by 384 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Blockade of phosphodiesterase 5 by sildenafil reduces tumor growth and potentiates tumor‐killing ability of cisplatin in vivo against T cell lymphoma: Implication of modulated apoptosis, reactive oxygen species homeostasis, glucose metabolism, and pH regulation;Environmental Toxicology;2023-12-07

2. Sirtuins and Metabolism Biomarkers in Relapsing-Remitting and Secondary Progressive Multiple Sclerosis: a Correlation Study with Clinical Outcomes and Cognitive Impairments;Molecular Neurobiology;2023-11-23

3. The antagonist of β-adrenergic receptor propranolol inhibits T cell lymphoma growth and enhances antitumor efficacy of cisplatin in vivo: A role of modulated apoptosis, glucose metabolism, pH regulation, and antitumor immune response;International Immunopharmacology;2023-11

4. Epinephrine facilitates the growth of T cell lymphoma by altering cell proliferation, apoptosis, and glucose metabolism;Chemico-Biological Interactions;2023-01

5. Integrated Metabolic-Transcriptomic Network Identifies Novel Immunometabolic Modulations in Human Macrophages;2023